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human 1 cdna microarray slides  (Agilent technologies)


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    Agilent technologies human 1 cdna microarray slides
    Human 1 Cdna Microarray Slides, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+microarray+slides/pmc02648774-120-9-14
    Average 90 stars, based on 1 article reviews
    human 1 cdna microarray slides - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Synovial fluid transcriptome dynamics in osteoarthritis progression: Implications in pathogenesis
    Article Snippet: Following hybridization, the array slides were washed and scanned with an Agilent SureScan High-Resolution DNA Microarray Scanner.

    Article Title: Role of ABCB1 and ABCB4 in renal and biliary excretion of perfluorooctanoic acid in mice
    Article Snippet: Cyanine-labeled cDNA was synthesized from the total RNA samples and hybridized to oligo DNA microarray slides (Whole Mouse Genome 4x44k; Agilent Technologies Japan).

    Microarray:

    Article Title: MicroRNAs in the Pathogenesis of Preeclampsia—A Case-Control In Silico Analysis
    Article Snippet: After hybridization, microarray slides were washed for 1 min at room temperature with GE wash buffer 1 (Agilent) and 1 min with 37 °C GE wash buffer 2 (Agilent) and then dried immediately by brief centrifugation. .. Slides were scanned immediately after washing on an Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 1 × 44k array slides (scan area of 61 × 21.6 mm 2 ; scan resolution of 10 μm; dye channel set to Green, and Green PMT was set to 100%). .. The scanned images were analyzed using Feature Extraction Software 9.1 (Agilent).

    Article Title: MicroRNAs in the Pathogenesis of Preeclampsia-A Case-Control In Silico Analysis.
    Article Snippet: After hybridization, microarray slides were washed for 1 min at room temperature with GE wash buffer 1 (Agilent) and 1 min with 37 ◦C GE wash buffer 2 (Agilent) and then dried immediately by brief centrifugation. .. Slides were scanned immediately after washing on an Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 1 × 44k array slides (scan area of 61 × 21.6 mm2; scan resolution of 10 μm; dye channel set to Green, and Green PMT was set to 100%). .. The scanned images were analyzed using Feature Extraction Software 9.1 (Agilent).

    Article Title: Sex differences in the tumor promoting effects of tobacco smoke in a cRaf transgenic lung cancer disease model.
    Article Snippet: The Agilent mouse miRNA microarray (Release 12.0, catalogue ID G4472B) contains 612 mouse mature miRNAs (https:// www. agile nt. com/ cs/ libra ry/ userm anuals/ public/ G4170- 90011. pdf). .. We scanned the hybridized microarray slides with an Agilent DNA Microarray Scanner G2505C and analyzed the data with the Agilent ScanControl version 8.1.3 software. .. We processed the scanned TIFF images numerically, applied QC tools and corrected for background and outlier pixels with the Agilent Feature Extraction Software version 10.7.7.1.

    Article Title: RENEB Inter-Laboratory Comparison 2021: The Gene Expression Assay
    Article Snippet: 200 ng total RNA was transcribed into cDNA with an oligo-dT primer, followed by transcription to cRNA labeled with cyanine 3-CTP (Quick-Amp Labeling Kit, One-color, Agilent). cRNA purification was performed with the RNeasy Mini Kit (Qiagen) and dye incorporation and cRNA yields were measured with the NanoDrop-1000 spectrophotometer (Thermofisher). .. Labeled cRNA samples were applied on the DNA microarray slides (44k whole human genome, G4112F, Agilent). .. For hybridization, DNA microarrays were placed into a hybridization oven (Agilent) at 65°C for 17 h. After hybridization, DNA microarrays were washed, and slides were immediately scanned with the Microarray Scanner (G2505 B, Agilent) as recommended by Agilent.

    Article Title: Molecular Events in Immune Responses to Sublingual Influenza Vaccine with Hemagglutinin Antigen and Poly(I:C) Adjuvant in Nonhuman Primates, Cynomolgus Macaques
    Article Snippet: .. The labeled cRNA was subsequently fragmented using the Gene Expression Hybridization Kit (Agilent Technologies, Santa Clara, CA, USA) and then set onto Whole Human Genome DNA Microarray 4× 44K v2 slides (Agilent Technologies, Santa Clara, CA, USA). .. After hybridization at 65 °C for 17 h, the slides were cleansed using Gene Expression Wash Buffers 1 and 2 (Agilent Technologies) according to the instructions provided by the manufacturer.

    Article Title: Gene Expression Profile Analysis of the Molecular Mechanism of HOXD10 Regulation of Epithelial Ovarian Cancer Cells
    Article Snippet: .. The slides were scanned using a DNA Microarray Scanner (Model G2505C, Agilent Technologies, Santa Clara, CA, USA). .. The intensity of Cy5 fluorescence for each spot was analyzed by GenePix 4.1 software (Molecular Devices, Sunnyvale, CA, USA).

    Software:

    Article Title: Sex differences in the tumor promoting effects of tobacco smoke in a cRaf transgenic lung cancer disease model.
    Article Snippet: The Agilent mouse miRNA microarray (Release 12.0, catalogue ID G4472B) contains 612 mouse mature miRNAs (https:// www. agile nt. com/ cs/ libra ry/ userm anuals/ public/ G4170- 90011. pdf). .. We scanned the hybridized microarray slides with an Agilent DNA Microarray Scanner G2505C and analyzed the data with the Agilent ScanControl version 8.1.3 software. .. We processed the scanned TIFF images numerically, applied QC tools and corrected for background and outlier pixels with the Agilent Feature Extraction Software version 10.7.7.1.

    Labeling:

    Article Title: RENEB Inter-Laboratory Comparison 2021: The Gene Expression Assay
    Article Snippet: 200 ng total RNA was transcribed into cDNA with an oligo-dT primer, followed by transcription to cRNA labeled with cyanine 3-CTP (Quick-Amp Labeling Kit, One-color, Agilent). cRNA purification was performed with the RNeasy Mini Kit (Qiagen) and dye incorporation and cRNA yields were measured with the NanoDrop-1000 spectrophotometer (Thermofisher). .. Labeled cRNA samples were applied on the DNA microarray slides (44k whole human genome, G4112F, Agilent). .. For hybridization, DNA microarrays were placed into a hybridization oven (Agilent) at 65°C for 17 h. After hybridization, DNA microarrays were washed, and slides were immediately scanned with the Microarray Scanner (G2505 B, Agilent) as recommended by Agilent.

    Article Title: Molecular Events in Immune Responses to Sublingual Influenza Vaccine with Hemagglutinin Antigen and Poly(I:C) Adjuvant in Nonhuman Primates, Cynomolgus Macaques
    Article Snippet: .. The labeled cRNA was subsequently fragmented using the Gene Expression Hybridization Kit (Agilent Technologies, Santa Clara, CA, USA) and then set onto Whole Human Genome DNA Microarray 4× 44K v2 slides (Agilent Technologies, Santa Clara, CA, USA). .. After hybridization at 65 °C for 17 h, the slides were cleansed using Gene Expression Wash Buffers 1 and 2 (Agilent Technologies) according to the instructions provided by the manufacturer.

    Gene Expression:

    Article Title: Molecular Events in Immune Responses to Sublingual Influenza Vaccine with Hemagglutinin Antigen and Poly(I:C) Adjuvant in Nonhuman Primates, Cynomolgus Macaques
    Article Snippet: .. The labeled cRNA was subsequently fragmented using the Gene Expression Hybridization Kit (Agilent Technologies, Santa Clara, CA, USA) and then set onto Whole Human Genome DNA Microarray 4× 44K v2 slides (Agilent Technologies, Santa Clara, CA, USA). .. After hybridization at 65 °C for 17 h, the slides were cleansed using Gene Expression Wash Buffers 1 and 2 (Agilent Technologies) according to the instructions provided by the manufacturer.

    Hybridization:

    Article Title: Molecular Events in Immune Responses to Sublingual Influenza Vaccine with Hemagglutinin Antigen and Poly(I:C) Adjuvant in Nonhuman Primates, Cynomolgus Macaques
    Article Snippet: .. The labeled cRNA was subsequently fragmented using the Gene Expression Hybridization Kit (Agilent Technologies, Santa Clara, CA, USA) and then set onto Whole Human Genome DNA Microarray 4× 44K v2 slides (Agilent Technologies, Santa Clara, CA, USA). .. After hybridization at 65 °C for 17 h, the slides were cleansed using Gene Expression Wash Buffers 1 and 2 (Agilent Technologies) according to the instructions provided by the manufacturer.



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    Image Search Results


    Results from primary chondrocytes 48 hr after seeding on stiff (100 kPa) or soft (0.5–1 kPa) ECM. ( a ) Microarray profiling of Wnt/β-catenin pathway transcripts. Results are normalized by median scaling using Rosetta Resolver System software. ( b ) Wnt1 and Wnt3a levels were analyzed by western blotting. ( c ) Total and phosphorylated ERK1/2 levels were analyzed by western blotting. ( d ) Axin2, CD44, and ( e ) phosphorylated GSK3β levels were analyzed by western blotting. ( f ) Total and phosphorylated β−catenin levels were analyzed by western blotting. ( g ) β−catenin levels in nucleus and cytoplasm were analyzed by western blotting. ( h ) Total and ( i ) activated β-catenin levels and distribution in chondrocytes 2 hr after seeding on stiff or soft ECM were analyzed by in situ fluorescence staining. ( j ) β-catenin and wnt1 levels in chondrocytes 48 hr after seeding on the Matrigel-coated PAAM were analyzed by western blotting. ( k ) β-catenin and wnt1 levels in chondrocytes 48 hr after seeding on the ColII-coated PAAM were analyzed by western blotting. Western results were from 3 independent experiments for each individual protein, with blots exemplifying one experiment and the bar graphs showing the combined results of 3 experiments on stiff matrix expressed as percentages (mean ± SEM) of the corresponding results on the soft matrix. GAPDH was used to normalize for equal loading. *P < 0.05, **P < 0.01. n.s. stands for not statistically significant.

    Journal: Scientific Reports

    Article Title: Extracellular matrix stiffness dictates Wnt expression through integrin pathway

    doi: 10.1038/srep20395

    Figure Lengend Snippet: Results from primary chondrocytes 48 hr after seeding on stiff (100 kPa) or soft (0.5–1 kPa) ECM. ( a ) Microarray profiling of Wnt/β-catenin pathway transcripts. Results are normalized by median scaling using Rosetta Resolver System software. ( b ) Wnt1 and Wnt3a levels were analyzed by western blotting. ( c ) Total and phosphorylated ERK1/2 levels were analyzed by western blotting. ( d ) Axin2, CD44, and ( e ) phosphorylated GSK3β levels were analyzed by western blotting. ( f ) Total and phosphorylated β−catenin levels were analyzed by western blotting. ( g ) β−catenin levels in nucleus and cytoplasm were analyzed by western blotting. ( h ) Total and ( i ) activated β-catenin levels and distribution in chondrocytes 2 hr after seeding on stiff or soft ECM were analyzed by in situ fluorescence staining. ( j ) β-catenin and wnt1 levels in chondrocytes 48 hr after seeding on the Matrigel-coated PAAM were analyzed by western blotting. ( k ) β-catenin and wnt1 levels in chondrocytes 48 hr after seeding on the ColII-coated PAAM were analyzed by western blotting. Western results were from 3 independent experiments for each individual protein, with blots exemplifying one experiment and the bar graphs showing the combined results of 3 experiments on stiff matrix expressed as percentages (mean ± SEM) of the corresponding results on the soft matrix. GAPDH was used to normalize for equal loading. *P < 0.05, **P < 0.01. n.s. stands for not statistically significant.

    Article Snippet: Microarray analyses were performed using commercial Mouse cDNA Microarray slides (Phalanx Biotech Group; Hsinchu, Taiwan) according to the manufacturer’s instructions.

    Techniques: Microarray, Software, Western Blot, In Situ, Fluorescence, Staining